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BLAST by Ian Korf
Book DescriptionSequence similarity is a powerful tool for discovering biological function. Just as the ancient Greeks used comparative anatomy to understand the human body and linguists used the Rosetta stone to decipher Egyptian hieroglyphs, today we can use comparative sequence analysis to understand genomes. BLAST (Basic Local Alignment Search Tool), is a sophisticated software package for rapid searching of nucleotide and protein databases. It is one of the most important software packages used in sequence analysis and bioinformatics. Most users of BLAST, however, seldom move beyond the program's default parameters, and never take advantage of its full power. BLAST is the only book completely devoted to this popular suite of tools. It offers biologists, computational biology students, and bioinformatics professionals a clear understanding of BLAST as well as the science it supports. This book shows you how to move beyond the default parameters, get specific answers using BLAST, and how to interpret your results. The book also contains tutorial and reference sections covering NCBI-BLAST and WU-BLAST, background material to help you understand the statistics behind BLAST, Perl scripts to help you prepare your data and analyze your results, and a wealth of tips and tricks for configuring BLAST to meet your own research needs. Some of the topics covered include: * BLAST basics and the NCBI web interface * How to select appropriate search parameters * BLAST programs: BLASTN, BLASTP, BLASTX, TBLASTN, TBLASTX, PHI-BLAST, and PSI BLAST * Detailed BLAST references, including NCBI-BLAST and WU-BLAST * Understanding biological sequences * Sequence similarity, homology, scoring matrices, scores, and evolution * Sequence Alignment * Calculating BLAST statistics * Industrial-strength BLAST, including developing applications with Perl and BLASTBLAST is the only comprehensive reference with detailed, accurate information on optimizing BLAST searches for high-throughput sequence analysis. This is a book that any biologist should own.
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Book DetailsISBN: 9780596002992
(233mm x 178mm x 19mm)
Imprint: O'Reilly Media, Inc, USA
Publisher: O'Reilly Media, Inc, USA
Publish Date: 8-Aug-2003
Country of Publication: United States
Books By Author Ian Korf
UNIX and Perl to the Rescue!, Paperback (July 2012)» View all books by Ian Korf
An accessible guide to learning the key features of Unix and Perl, written with the non-programmer in mind.
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Author Biography - Ian Korf
Ian Korf is from Washington University, where he works closely with Warren Gish, one of the creators of BLAST. Mark Yandell is from Celera Genomics. He, and the company he works for, make extensive use of BLAST in their gene sequencing and gene discovery efforts. He is an expert at using BLAST, and represents most of the biologists using BLAST today. Joseph Bedell is from Incyte Genomics. He, and the company he works for, make extensive use of BLAST in their gene sequencing and gene discovery efforts. He is an expert at using BLAST, and represents most of the biologists using BLAST today.
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