A Practical Approach
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Epitope Mapping by Olwyn Westwood
Book DescriptionEpitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific eptiope. Investigation of carbohydrates can also be useful to eptitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.
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Book DetailsISBN: 9780199636532
(195mm x 253mm x 21mm)
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 22-Mar-2001
Country of Publication: United Kingdom
Books By Author Olwyn Westwood
How to Assess Students and Trainees in Medicine and Health, Paperback (May 2013)
Do you want to improve standards of practice? Do you know how to construct examinations so that they are fit for purpose? Can you give constructive feedback to aid development? How to Assess Students and Trainees in Medicine and Health will help you develop these vital skills and much more.
Practical Immunology, Paperback (February 2002)
Offers an overview of the methodology of immunology. This book explains techniques from first principles and describes basic forms of apparatus. It also includes useful hints and tips.
Epitope Mapping, Paperback (March 2001)» View all books by Olwyn Westwood
An epitope is a structural region of an antigen that is recognized by an antibody and is therefore central to the immune response. Epitope Mapping describes the various methods for their location and characterization. This process is an essential part of developing non-pathogenic vaccines.
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