Epitope Mapping by Olwyn Westwood
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Epitope Mapping
By Olwyn Westwood

Epitope Mapping

A Practical Approach

Edited by See other recent books by Olwyn Westwood See other recent books by Frank C. Hay
Format: Hardback

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Epitope Mapping by Olwyn Westwood

Book Description

Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific eptiope. Investigation of carbohydrates can also be useful to eptitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.

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Book Details

ISBN: 9780199636532
ISBN-10: 0199636532
Format: Hardback
(195mm x 253mm x 21mm)
Pages: 302
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 22-Mar-2001
Country of Publication: United Kingdom

Other Editions...


Books By Author Olwyn Westwood

How to Assess Students and Trainees in Medicine and Health by Olwyn Westwood How to Assess Students and Trainees in Medicine and Health, Paperback (May 2013)

Do you want to improve standards of practice? Do you know how to construct examinations so that they are fit for purpose? Can you give constructive feedback to aid development? How to Assess Students and Trainees in Medicine and Health will help you develop these vital skills and much more.

Practical Immunology by Olwyn Westwood Practical Immunology, Paperback (February 2002)

Offers an overview of the methodology of immunology. This book explains techniques from first principles and describes basic forms of apparatus. It also includes useful hints and tips.

Epitope Mapping by Olwyn Westwood Epitope Mapping, Paperback (March 2001)

An epitope is a structural region of an antigen that is recognized by an antibody and is therefore central to the immune response. Epitope Mapping describes the various methods for their location and characterization. This process is an essential part of developing non-pathogenic vaccines.

» View all books by Olwyn Westwood

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Recent books by Olwyn Westwood close
How to Assess Students and Trainees in Medicine and Health by Olwyn Westwood
Practical Immunology by Olwyn Westwood
Epitope Mapping by Olwyn Westwood
»
Recent books by Frank C. Hay close
How to Assess Students and Trainees in Medicine and Health by Frank C. Hay
Practical Immunology by Frank C. Hay
Epitope Mapping by Frank C. Hay
Epitope Mapping by Frank C. Hay
»
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