Growth Factors and Receptors
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Growth Factors and Receptors by Ian McKay
Book DescriptionGrowth Factors and Receptors: A Practical Approach provides comprehensive protocols for studies of growth factors and their interactions with receptors. It covers a wide range from simple analytical techniques to sophisticated in vivo applications including: RT-PCR and immunocytochemistry for detection of growth factors and receptors; production and purification of recombinant growth factors and receptors; labelling of growth factors for binding studies; in vivo mutagenesis; the yeast two-hybrid assay of proteinprotein interactions; phage display of factors; application of factors to wound-healing processes using the gene gun; treatment of cancers with factor/toxin chimeras; and analysis of important factor domains using chimeric proteins. This book updates and extends the current literature and describes important novel approaches to the study of growth factors and their receptors, including the use of RNA aptamers as receptor antagonists, and the development of receptor superantagonists. It will be of tremendous value to both researchers and teachers, and, through an appendix that lists a large number of growth factors and receptors, will serve as a handy reference text.
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Book DetailsISBN: 9780199636464
(235mm x 157mm x 16mm)
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 2-Jul-1998
Country of Publication: United Kingdom
Books By Author Ian McKay
Growth Factors, Paperback (March 1993)» View all books by Ian McKay
Growth factors play an important role in the regulation of proliferation and differentiation of specific cell types. This is a guide to studying growth factors, from the search for and characterization of new activities, to "in vivo" analysis and molecular biology approaches such as DNA cloning.
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Author Biography - Ian McKay
Dr Ian A. McKay Senior Lecturer in Dermatology Centre for Cutaneous Research 2 Newark Street London E1 2AT Tel: 0171 295 7160 Fax: 0171 295 7171 Email: I.A.McKay@mds.qmw.ac.uk Dr Kenneth D. Brown Institute of Animal Physiology The Babraham Institute Babraham Hall Babraham, Cambridge CB2 4AT Tel: 01223 833312 Fax: 01223 833511 Email: email@example.com
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