Kinetic Analysis of Macromolecules
A Practical Approach
Edited by Kenneth Johnson
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Kinetic Analysis of Macromolecules by Kenneth Johnson
Book DescriptionModern enzymology can no longer rely upon indirect inferences derived by steady state kinetic analysis because these are often misleading. Rather, in the era of 'New Enzymology', reactions at the active sites of enzyme are monitored by direct measurement of individual steps in the sequence to establish the pathway of reactions occurring. These quantitative methods can be directly related to high resolution structural information and define the underlying kinetic and thermodynamic basis for catalysis. The goal of a comprehensive kinetic analysis is to identify each step in the reaction pathway, define the rate and equilibrium constants for each step, identify all conformational and chemical intermediates, identify the rate-limiting steps, and ultimately establish the basis for enzymatic specificity and efficiency. This volume contains chapters contributed by the leading experts in the application of modern kinetic methods to complex problems in the study of catalysis by both proteins and RNA catalysts. Each chapter focuses on a unique system and the novel methods used to obtain definitive data that can be directly applied to structural information, giving the much-needed practical details that are often omitted in the general literature. Readers will benefit greatly from the richness of each chapter in describing what is most important in solving the most difficult problems in modern enzymology.
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Book DetailsISBN: 9780198524939
(254mm x 195mm x 20mm)
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 25-Sep-2003
Country of Publication: United Kingdom
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Author Biography - Kenneth Johnson
Kenneth A. Johnson Roger Williams Centennial Professor of Biochemistry Institute for Cell & Molecular Biology, University of Texas, A4800 MBB 3.122, Austin, TX 78735 512-471-0434 512-471-0435 firstname.lastname@example.org Roger Williams Centennial Professor of Biochemistry The University of Texas at Austin Recipient of Pfizer Award in Enzyme Chemistry, 1989 Founder and President of KinTek Corporation, manufacturer of stopped-flow and quench-flow instruments for fast reaction kinetics. Editorial Board Member, Journal of Biological Chemistry
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