Protein Purification Applications by E. L. V. Harris
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Protein Purification Applications
By E. L. V. Harris

Protein Purification Applications

A Practical Approach

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Format: Hardback

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Protein Purification Applications by E. L. V. Harris

Book Description

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

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Book Details

ISBN: 9780199636723
ISBN-10: 0199636729
Format: Hardback
(253mm x 195mm x 15mm)
Pages: 182
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 25-Jan-2001
Country of Publication: United Kingdom

Other Editions...


Books By Author E. L. V. Harris

Protein Purification Techniques by E. L. V. Harris Protein Purification Techniques, Hardback (January 2001)

The protein purification Practical Approach books contain detailed practical information on the separation and purification of proteins. Protein Purification Techniques covers unit operations and analytical techniques in detail, while Protein Purification Applications has details of protein purification from a selected number of typical sources.

Protein Purification Applications by E. L. V. Harris Protein Purification Applications, Paperback (January 2001)

The protein purification Practical Approach books contain detailed practical information on the separation and purification of proteins. Protein Purification Techniques covers unit operations and analytical techniques in detail, while Protein Purification Applications has details of protein purification from a selected number of typical sources.

» View all books by E. L. V. Harris

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Protein Purification Techniques by E. L. V. Harris
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