The Proteome Revisited
Theory and Practice of All Relevant Electrophoretic Steps
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Proteome Revisited by Pier Giorgio Righetti
Book DescriptionThe book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.
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Book DetailsISBN: 9780444505262
(248mm x 171mm x 25mm)
Imprint: Elsevier Science Ltd
Publisher: Elsevier Science & Technology
Publish Date: 1-Oct-2001
Country of Publication: United Kingdom
Books By Author Pier Giorgio Righetti
Low-Abundance Proteome Discovery, Paperback (May 2013)View all books by Pier Giorgio Righetti
Addresses the critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. This book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries.
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