Description - The Proteome Revisited by Pier Giorgio Righetti
This text deals with the theory and practice of all electrophoretic steps leading to proteome analysis, that is, isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. The book also combines practice with theory, by giving a detailed treatment advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients.
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Elsevier Science Ltd
Publisher: Elsevier Science & Technology
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